Application

Western blot: 1:1,000-1:10,000 colorimetric; 1:1,000-1:30,000 ECL. Immunocytochemistry: 1:100-1:400 ELISA: 1:100-1:500 for coating; 1:1,000-1:30,000 for primary.Immunofluorescence-suitable Immunoprecipitation: 2-5μg/mL in 300-500μL of cell lysate (150-300μg/mL total protein). Lysis buffer 0.5% triton X-100 with proteinase inhibitors. Use high quality protein A beads or anti-rabbit conjugated beads for best results. Run bead-only (no T7 antibody) controls to mark bands related to protein A breakdown products. Optimal working dilutions must be determined by the end user.

Research Sub CategoryEpitope Tags

Research CategoryEpitope Tags & General Use

Anti-T7 Epitope Tag Antibody, T7 (MASMTGGQQMG) detects level of T7 Epitope Tag & has been published & validated for use in IP, WB & IC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

MASMTGGQQMG (T7) conjugated to KLH

Epitope: T7 (MASMTGGQQMG)

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in PBS, pH 7.2 with 0.1% sodium azide.

Specificity

T7 (MASMTGGQQMG). Rabbits were immunized with T7 (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Storage and Stability

Maintain at 2-8°C in undiluted for up to 6 months from date of receipt.